Table 1

Quantitation of threshold-integrated density of proinsulin, insulin, and Zn2+ staining in RW and WT Tg+ and Tg− mice fed an HFD or ND

ZP1InsulinProinsulin
RW HFD Tg+458 ± 83a1,c1,d1479 ± 52.6a2,c2,d2199 ± 20a3,c3
RW HFD Tg−782 ± 87343 ± 30.6284 ± 78
RW ND Tg+804 ± 120b1121.4 ± 18b2116 ± 8.9b3
RW ND Tg−620 ± 11650.9 ± 9.6240 ± 35
WT HFD Tg+828 ± 138e1,f148.1 ± 9.6e2,f2204 ± 21.3e3
WT HFD Tg−466 ± 5780.5 ± 9.2137 ± 16.4
WT ND Tg+301 ± 5631.2 ± 6.4205 ± 8.5
WT ND Tg−646 ± 7831.2 ± 11206 ± 33
  • Values are the average × 104 ± SEM in relative fluorescence units of threshold-integrated density from 3–4 independent experiments (n = 15–25).

  • a1,a2,a3 indicate a significant difference between ZnT8RW Tg+ and Tg− mice fed an HFD in pancreatic ZP1, insulin, and proinsulin staining, respectively.

  • b1,b2,b3 indicate a significant difference between ZnT8RW and ZnT8WT Tg+ mice fed a ND in pancreatic ZP1, insulin, and proinsulin staining, respectively.

  • c1,c2,c3 indicate a significant difference between ZnT8RW Tg+ mice fed an HFD vs. ND in pancreatic ZP1, insulin, and proinsulin staining, respectively.

  • d1,d2 indicate a significant difference between ZnT8RW and ZnT8WT Tg+ mice fed an HFD in pancreatic ZP1, insulin, and proinsulin staining, respectively.

  • e1,e2,e3 indicate a significant difference between ZnT8WT Tg+ and Tg− mice fed an HFD in pancreatic ZP1, insulin, and proinsulin staining, respectively.

  • f1,f2 indicate a significant difference between ZnT8WT Tg+ and Tg− mice fed a ND in pancreatic ZP1 and insulin staining, respectively.