Table 1

Glucose-regulated miRNAs in mouse pancreatic islets

	Fold change	P value	Ct G3	Ct G11
Upregulated miRNAs at low glucose (G3 vs. G11)
mmu-miR-708-5p	6.7	0.0014	29.0 ± 0.13	31.9 ± 0.39
mmu-miR-339-5p	2.5	0.0032	31.8 ± 0.50	33.1 ± 0.30
mmu-miR-451a	2.5	0.0377	34.0 ± 0.06	34.5 ± 0.39
mmu-miR-224-5p	2.1	0.0433	34.2 ± 0.20	35.4 ± 0.29
mmu-miR-129-2-3p	2.0	0.0062	28.7 ± 0.48	30.0 ± 0.05
mmu-miR-467a-5p	1.9	0.0323	31.8 ± 0.25	33.0 ± 0.27
mmu-miR-151-3p	1.9	0.0250	30.1 ± 0.05	31.3 ± 0.06
Downregulated miRNAs at low glucose (G3 vs. G11)
rno-miR-346	−3.2	0.0017	33.9 ± 0.28	32.5 ± 0.15
mmu-miR-138-5p	−2.5	0.0300	35.0 ± 0.20	34.0 ± 0.32
mmu-miR-33-5p	−2.1	0.0246	29.7 ± 0.77	28.7 ± 0.12
mmu-miR-541-5p	−1.8	0.0297	28.9 ± 0.39	28.3 ± 0.10
mmu-miR-370-3p	−1.6	0.0118	30.8 ± 0.39	30.3 ± 0.08

The expression of miRNAs was analyzed by a high-throughput screening of miRNA expression using quantitative PCR 384-well panels. RNA was extracted from mouse pancreatic islets cultured at 2.8 mmol/L glucose (G3) and at 11.1 mmol/L glucose (G11) for 2.5 days. Ct G3 and Ct G11 values indicate the cycle threshold ± SEM from qPCR analysis. cDNA synthesis for each sample was performed using 20 ng of total RNA. The listed miRNAs are those whose expression is significantly different between the G3 and G11 conditions. Statistical significance was determined by multiple t tests corrected for multiple comparisons using the Holm-Sidak method (P < 0.05).