Table 1

Sources of virus and antibody preparations used

Virus/antibody; titer/dilutionApplicationSourceReferences
AAV2-FLEX-hM3Dq:mCherry; 6.1 × 1012Activation of Cre-expressing PPG neuronsUNC Vector Core, Chapel Hill, NCpAAV-hSyn-DIO-hM3D(Gq)-mCherry was a gift from Bryan Roth (49)
AAV8-FLEX-hM3Dq:mCherry; 4 × 1012Activation of Cre-expressing PPG neuronsVVF, ZNZ, Zurich, SwitzerlandpAAV-hSyn-DIO-hM3D(Gq)-mCherry was a gift from Bryan Roth (49)
AAV8-mCherry-FLEX-DTA; 3.3 × 1012Ablation of Cre-expressing PPG neuronsUNC Vector Core, Chapel Hill, NCpAAV-mCherry-flex-dtA was a gift from Naoshige Uchida
AAV2-FLEX-hM4Di:mCherry; 6.4 × 1012Inhibition of Cre-expressing PPG neuronsVVF, ZNZ, Zurich, SwitzerlandpAAV-hSyn-DIO-hM4D(Gi)-mCherry was a gift from Bryan Roth (49)
AAV1/2-FLEX-Perceval; titer not determinedControl for viral transductionMade in-housepAAV-FLEX-empty was a gift from Bill Wisden (50) pShuttleCMV-Perceval was a gift from Guy Rutter
AAV8-FLEX-EGFP; 8 × 1012Control for viral transductionVVF, ZNZ, Zurich, SwitzerlandpAAV-hSyn-DIO-EGFP was a gift from Bryan Roth
PRSx8-AlstR-EGFP-LV; 1 × 1010Identification of Phox2b neuronsSergei Kasparov(26)
Chicken anti-GFP, Alexa Fluor 488 goat anti-chicken; 1:1,000GCaMP3, EGFP, YFPAB13970; Abcam/#A-11039; Invitrogen(18)
Rabbit anti-dsRed, Cy3 sheep anti-rabbit; 1:1,000mCherry, tdRFP#632496; TaKaRa Bio Inc./#C2306; Sigma
Rabbit anti-cFOS; 1:500 Alexa Fluor 488 goat anti-rabbit; 1:1,000cFOS#ABE457; Merck/#A-11008; Invitrogen
  • UNC, University of North Carolina; VVF, Viral Vector Facility; ZNZ, Neuroscience Center Zurich.