Table 1

Differential expression analysis of miRNA abundance in exoRNA

miRNAqPCR panelIndividual miRNA assay relative expression level (control vs. diabetes)
Direction of changeFold change from NGTP
hsa-miR-20b-5pUp5.30.0441.52 ± 0.27*
hsa-miR-532-3pUp3.20.0081.29 ± 0.15
hsa-miR-150-5pUp1.50.0241.65 ± 0.19*
hsa-miR-502-3pDown3.00.0341.07 ± 0.20
hsa-miR-363-3pDown1.80.0381.24 ± 0.19
hsa-miR-30d-5pDown1.20.0431.23 ± 0.11
  • Data from the PCR panel are reported as mean values of fold changes for men with type 2 diabetes vs. men with normal glucose tolerance (control) and each P value of n = 4 each group. Six miRNAs showing significantly altered expression in exoRNAs derived from men with type 2 diabetes were confirmed by individual miRNA qRT-PCR assays. Shown are the relative levels (mean ± SEM for men with type 2 diabetes vs. normal glucose tolerance [control]) for n = 20 control subjects and n = 21 subjects with type 2 diabetes. NGT, normal glucose tolerance.

  • *P < 0.05 comparing control subjects vs. subjects with diabetes.