Table 3

Summary of GSEA

Annotated cellular functionOverlapping genes (n)PFDR (q)
Downregulated by miR-20b-5p
 Interferon_alpha_response96<0.001<0.001
 Interferon_gamma_response195<0.001<0.001
 TNFA_signaling_via_NFKB198<0.001<0.001
 IL2_STAT5_signaling194<0.001<0.001
 IL6_JAK_STAT3_signaling83<0.05<0.05
Upregulated by miR-20b-5p
 MYC_targets_V1195<0.001<0.01
 Cholesterol_homeostasis74<0.001<0.001
 MTORC1_signaling198<0.001<0.001
 G2M_checkpoint196<0.001<0.001
 E2F_targets192<0.001<0.001
 Unfolded_protein_response111<0.001<0.01
 Androgen_response98<0.001<0.01
 Estrogen_response_early196<0.001<0.05
 MYC_targets_V254<0.05<0.05
 Fatty_acid_metabolism153<0.050.077288
 PI3K_AKT_MTOR_signaling1040.0794390.133628
  • GSEA analysis was performed on the ranked genes according to the ratios of transcripts from mimic control– and miR-20b-5p–transfected human skeletal muscle cells. Fourteen gene sets with a P value of <0.05 and a false discovery rate (FDR) of <0.05 were considered significant comparing miR-20b-5p–transfected cells with mimic miRNA–transfected control cells. Of these, five gene sets associate with the miR-20b-5p–induced downregulated genes and nine gene sets are associated with miR-20b-5p–induced upregulated genes.