Table 4

miR-20b-5p–induced downregulated genes in human skeletal muscle cells

Gene symbolGene nameMicroarrayPredicted miR-20b-5p target siteIndividual miRNA assay relative expression level (transfected vs. NC)
Fold change (transfected vs. NC)P
CYBRD1Cytochrome b reductase 14.10.0023Yes0.15 ± 0.039**
TBC1D2TBC1 domain family, member 23.00.0049Yes0.30 ± 0.038**
AKTIPAKT interacting protein2.70.0022Yes0.37 ± 0.020**
RNH1RNase/angiogenin inhibitor 12.60.0028YesND
GINM1Glycoprotein integral membrane 12.60.000013Yes0.37 ± 0.0064**
CFL2Cofilin 2 (muscle)2.50.0016Yes0.41 ± 0.084*
  • Data of relative expression level by individual miRNA assay are means ± SEM of n = 3. The microRNA.org resource was used for prediction of miR-20b-5p target sites. NC, mimic miRNA–transfected; ND, no data.

  • *P < 0.05;

  • **P < 0.01 comparing miR-20b-5p–transfected cells with mimic miRNA–transfected control cells.