Table 2

Physiologic parameters across 4 months of acclimation

	Month 1: 24°C		Month 2: 19°C		Month 3: 24°C			Month 4: 27°C		P_trend
	24°C	19°C	24°C	19°C	24°C		19°C	24°C	19°C	24°C	19°C
Total EE (kcal)	2,472 ± 180	2,624 ± 198^a	2,366 ± 358	2,543 ± 410^a	2,400 ± 252		2,555 ± 346^a	2,341 ± 255	2,505 ± 322^a	0.45	0.46
Respiratory quotient	0.84 ± 0.03	0.84 ± 0.01	0.84 ± 0.02	0.83 ± 0.02	0.85 ± 0.02		0.84 ± 0.02	0.84 ± 0.03	0.85 ± 0.02	0.72	0.47
Total activity (units)	8.4 ± 1.6	8.4 ± 2.8	7.6 ± 2.9	7.1 ± 2.4	6.8 ± 3.7		7.1 ± 4.1	7.9 ± 5.0	7.2 ± 4.7	0.54	0.38
Surface electromyography (×10⁻⁶ RMS)	2.8 ± 0.4	2.5 ± 1.3	2.7 ± 0.3	2.6 ± 0.2	2.8 ± 0.5		2.7 ± 0.2	2.8 ± 0.3	2.6 ± 0.4	0.98	0.83
CIT (%)	6.2 ± 4.1		7.4 ± 3.1		6.2 ± 3.9			6.8 ± 3.2		0.16
DIT (%)	10.3 ± 13.1	33.4 ± 18.2	19.0 ± 15.4	42.2 ± 17.4^a^,^b	19.1 ± 16.0	37.1 ± 19.6^a		22.5 ± 11.0	34.4 ± 19.2^a	0.30	0.36

Data are means ± SD. At the end of each testing month, subjects underwent acute thermometabolic evaluation at either 24°C or 19°C. RMS, root mean square.
^aP < 0.05 compared with 24°C during acute thermometabolic evaluation each month;
^bP < 0.05 (month 1 vs. 2) compared with matching measurement at same temperature performed at respective months as indicated.