Table 1

Antibodies used in this study

Antibody targetSourceDilution (application)
VPS41 (mouse)Santa Cruz Biotechnology (D-12)1:100 (Western)
Tubulin (mouse)DSHB (E-7)1:500 (Western)
Actin (mouse)DSHB (JLA20)1:500 (Western)
Synaptophysin (mouse)Synaptic Systems (MMS-618R)1:2,000 (Western)
PC1/3 (rabbit)Richard Mains (gift)1:500 (Western)
TFE3 (rabbit)Cell Signaling Technology (14,779)1:500 (IF)
Insulin (mouse)Sigma-Aldrich (K36AC10)1:1,000 (IF)
HA (rat)Sigma-Aldrich (3F10)1:1,000 (IF)
Phogrin (rabbit)Zippy from John Hutton1:100 (Western)
Proinsulin (mouse)DSHB (GS-9A8)1:500 (IF)
  • DSHB, Developmental Studies Hybridoma Bank; IF, immunofluorescence.