Table 1

RAS metabolites in the lungs of young (9 weeks of age) male and female C57BL/6N mice, aged mice (44 weeks of age), and male aged, DM-HFD mice treated with vehicle or ramipril (10 mg/kg/day) or telmisartan (3 mg/kg/day) for 2 weeks

Ang II (1–8) (fmol/g)Ang 1–7 (fmol/g)Ang I (1–10) (fmol/g)Ang III (2–8) (fmol/g)Ang 1–5 (fmol/g)Ang IV (3–8) (fmol/g)
Male C57BL/6N
 Mouse 132.1<89.67.9<3<2
 Mouse 224.2<8<45.5<3<2
 Mouse 330.4<8<44.7<32.4
 Mouse 416.4<8<45.43.2<2
 Mouse 525.2<8<47.9<3<2
 Mean ± SD25.7 ± 6.7
Female C57BL/6N
 Mouse 613.3<8<4<4<3<2
 Mouse 727.9<8<412.74.22.7
 Mouse 828.9<8<412.1<3<2
 Mouse 917.8<8<47.4<3<2
 Mouse 1018.4<8<44.4<3<2
 Mean ± SD21.3 ± 6.8
Aged control
 Mouse 1173.5<8<421.4<33.3
 Mouse 1262.8<8<418.3<3<2
 Mouse 13142.6<87.940.37.16.4
 Mouse 1431.9<8<413.5<3<2
 Mouse 1564.6<8<417.2<3<2
 Mean ± SD75.1 ± 40.9a,b
DM-HFD + vehicle
 Mouse 16162.5<810.542.913.39.2
 Mouse 1722.5<8<46.3<32.1
 Mouse 1873.1<84.520.96.3<2
 Mouse 1977.5<89.915.48.52.3
 Mouse 2017.8<8<4<43.8<2
 Mean ± SD70.7 ± 58.3a,b
DM-HFD + ramipril
 Mouse 2123.9<826.75.511.1<2
 Mouse 2212.3<816.7<4<3<2
 Mouse 2325.7<845.3<4<3<2
 Mouse 242.2<8<4<4<3<2
 Mouse 2514.3<8<4<4<3<2
 Mean ± SD15.7 ± 9.6c,d
DM-HFD + telmisartan
 Mouse 268.4<8<4<4<3<2
 Mouse 275.7<8<4<4<3<2
 Mouse 2836.6<8<4<4<34.0
 Mouse 2924.6<8<4<4<32.2
 Mouse 30103.118.630.923.515.88.0
 Mean ± SD35.7 ± 39.7
  • a P < 0.05 vs. male C57BL/6N.

  • b P < 0.05 vs. female C57BL/6N.

  • c P < 0.05 vs. aged control.

  • d P < 0.05 vs. DM-HFD + vehicle by one-way ANOVA followed by Fisher least significant difference post hoc test.