RAS metabolites in the lungs of young (9 weeks of age) male and female C57BL/6N mice, aged mice (44 weeks of age), and male aged, DM-HFD mice treated with vehicle or ramipril (10 mg/kg/day) or telmisartan (3 mg/kg/day) for 2 weeks
| Ang II (1–8) (fmol/g) | Ang 1–7 (fmol/g) | Ang I (1–10) (fmol/g) | Ang III (2–8) (fmol/g) | Ang 1–5 (fmol/g) | Ang IV (3–8) (fmol/g) | |
|---|---|---|---|---|---|---|
| Male C57BL/6N | ||||||
| Mouse 1 | 32.1 | <8 | 9.6 | 7.9 | <3 | <2 |
| Mouse 2 | 24.2 | <8 | <4 | 5.5 | <3 | <2 |
| Mouse 3 | 30.4 | <8 | <4 | 4.7 | <3 | 2.4 |
| Mouse 4 | 16.4 | <8 | <4 | 5.4 | 3.2 | <2 |
| Mouse 5 | 25.2 | <8 | <4 | 7.9 | <3 | <2 |
| Mean ± SD | 25.7 ± 6.7 | – | – | – | – | – |
| Female C57BL/6N | ||||||
| Mouse 6 | 13.3 | <8 | <4 | <4 | <3 | <2 |
| Mouse 7 | 27.9 | <8 | <4 | 12.7 | 4.2 | 2.7 |
| Mouse 8 | 28.9 | <8 | <4 | 12.1 | <3 | <2 |
| Mouse 9 | 17.8 | <8 | <4 | 7.4 | <3 | <2 |
| Mouse 10 | 18.4 | <8 | <4 | 4.4 | <3 | <2 |
| Mean ± SD | 21.3 ± 6.8 | – | – | – | – | – |
| Aged control | ||||||
| Mouse 11 | 73.5 | <8 | <4 | 21.4 | <3 | 3.3 |
| Mouse 12 | 62.8 | <8 | <4 | 18.3 | <3 | <2 |
| Mouse 13 | 142.6 | <8 | 7.9 | 40.3 | 7.1 | 6.4 |
| Mouse 14 | 31.9 | <8 | <4 | 13.5 | <3 | <2 |
| Mouse 15 | 64.6 | <8 | <4 | 17.2 | <3 | <2 |
| Mean ± SD | 75.1 ± 40.9a,b | – | – | – | – | – |
| DM-HFD + vehicle | ||||||
| Mouse 16 | 162.5 | <8 | 10.5 | 42.9 | 13.3 | 9.2 |
| Mouse 17 | 22.5 | <8 | <4 | 6.3 | <3 | 2.1 |
| Mouse 18 | 73.1 | <8 | 4.5 | 20.9 | 6.3 | <2 |
| Mouse 19 | 77.5 | <8 | 9.9 | 15.4 | 8.5 | 2.3 |
| Mouse 20 | 17.8 | <8 | <4 | <4 | 3.8 | <2 |
| Mean ± SD | 70.7 ± 58.3a,b | – | – | – | – | – |
| DM-HFD + ramipril | ||||||
| Mouse 21 | 23.9 | <8 | 26.7 | 5.5 | 11.1 | <2 |
| Mouse 22 | 12.3 | <8 | 16.7 | <4 | <3 | <2 |
| Mouse 23 | 25.7 | <8 | 45.3 | <4 | <3 | <2 |
| Mouse 24 | 2.2 | <8 | <4 | <4 | <3 | <2 |
| Mouse 25 | 14.3 | <8 | <4 | <4 | <3 | <2 |
| Mean ± SD | 15.7 ± 9.6c,d | – | – | – | – | – |
| DM-HFD + telmisartan | ||||||
| Mouse 26 | 8.4 | <8 | <4 | <4 | <3 | <2 |
| Mouse 27 | 5.7 | <8 | <4 | <4 | <3 | <2 |
| Mouse 28 | 36.6 | <8 | <4 | <4 | <3 | 4.0 |
| Mouse 29 | 24.6 | <8 | <4 | <4 | <3 | 2.2 |
| Mouse 30 | 103.1 | 18.6 | 30.9 | 23.5 | 15.8 | 8.0 |
| Mean ± SD | 35.7 ± 39.7 | – | – | – | – | – |
a P < 0.05 vs. male C57BL/6N.
b P < 0.05 vs. female C57BL/6N.
c P < 0.05 vs. aged control.
d P < 0.05 vs. DM-HFD + vehicle by one-way ANOVA followed by Fisher least significant difference post hoc test.