Table 3

Causal mediation analysis on the significant associations between the lifestyle intervention and lean mass–related methylation (CpG) sites as mediators and lean mass (%) as outcome (ACME q value <0.05)

CpG siteGeneACME estimate of mediator CpG (95% CI)ACME q valueADE estimate (95% CI)Total effect (95% CI)Proportion mediated by CpG (95% CI)
cg07405330MOBP0.84 (0.35; 1.39)<0.0010.52 (−0.84; 1.89)1.35 (−0.09; 2.74)0.62 (−0.73; 3.58)
cg06480224KIAA20120.78 (0.19; 1.51)0.0130.57 (−0.97; 1.98)1.35 (−0.09; 2.74)0.58 (−1.11; 4.27)
cg11612786AC079135.1;GBX20.64 (0.13; 1.38)0.0130.71 (−0.66; 2.03)1.35 (−0.09; 2.74)0.47 (−0.58; 2.78)
cg209820520.53 (0.14; 1.11)0.0130.82 (−0.68; 2.18)1.35 (−0.09; 2.74)0.39 (−0.62; 2.63)
cg00154557DSE0.50 (0.08; 1.08)0.0210.86 (−0.64; 2.26)1.35 (−0.09; 2.74)0.37 (−0.58; 2.42)
cg13002044TMEM178B0.70 (0.14; 1.42)0.0210.65 (−0.73; 1.98)1.35 (−0.09; 2.74)0.52 (−0.85; 2.87)
cg18088415LSM20.70 (0.19; 1.41)0.0210.65 (−0.77; 2.07)1.35 (−0.09; 2.74)0.52 (−0.73; 3.42)
cg11594420TEX1010.53 (0.10; 1.18)0.0250.82 (−0.59; 2.15)1.35 (−0.09; 2.74)0.39 (−0.45; 2.84)
cg046783150.48 (0.08; 1.02)0.0280.87 (−0.56; 2.20)1.35 (−0.09; 2.74)0.36 (−0.64; 2.05)
cg081446750.70 (0.10; 1.42)0.0320.66 (−0.85; 2.20)1.35 (−0.09; 2.74)0.52 (−0.98; 3.35)
cg22454673HERC20.63 (0.16; 1.29)0.0320.73 (−0.71; 2.18)1.35 (−0.09; 2.74)0.46 (−0.77; 3.10)
cg04058675HUWE10.45 (0.08; 1.03)0.0330.90 (−0.46; 2.22)1.35 (−0.09; 2.74)0.34 (−0.48; 2.09)
cg03190725RP3–468B3.20.54 (0.10; 1.10)0.0350.81 (−0.67; 2.19)1.35 (−0.09; 2.74)0.40 (−0.65; 2.58)
cg06799721TARS0.52 (0.05; 1.27)0.0360.84 (−0.54; 2.07)1.35 (−0.09; 2.74)0.38 (−0.30; 2.00)
cg15157974DISC10.43 (0.04; 0.96)0.0400.92 (−0.50; 2.21)1.35 (−0.09; 2.74)0.32 (−0.24; 2.10)
cg26142132AAT0.45 (0.06; 0.96)0.0410.90 (−0.57; 2.40)1.35 (−0.09; 2.74)0.34 (−0.44; 2.26)
cg00354884ABR0.53 (0.03; 1.18)0.0470.83 (−0.62; 2.17)1.35 (−0.09; 2.74)0.39 (−0.45; 2.32)
  • Models adjusted for GWG (in kilograms), maternal BMI, GA (in weeks), and offspring sex. Based on 139 participants, lifestyle intervention, n = 92, and control, n = 47.

  • ACME, average causal mediator effect; ADE, average direct effect.