Table 1

Sequences of primers and oligonucleotides used for generation of constructs, mutagenesis, EMSA probes, ChIP, and quantitative real-time PCR

Primer/oligonucleotideSequence
Primers for constructs
 p(−2004 to +588)LucF1: 5′-CTAGCTAGCAACTGCCTCAGCTGCTTCAC-3′; R1: 5′-CCGCTCGAGAAGAAGGGGAGCTTGTGTGG-3′
 p(−2004 to +41)LucF1; R2: 5′-CCGCTCGAGGGGCTGCCGAAGAGCTTTAG-3′
 p(−1591 to +41)LucF2: 5′-CTAGCTAGCCAGCCAGTGACTCCAGTAAC-3′; R2
 p(−894 to +41)LucF3: 5′-CTAGCTAGCCACCATTGACCCACTAAACC-3′; R2
 p(−186 to +41)LucF4: 5′-CTAGCTAGCGCTGGCTGCACACACTTGAG-3′; R2
 p(−34 to +41)LucF5: 5′-CTAGCTAGCTTAGCAGACCCGCCTGCAAT-3′; R2
 p(−186 to +588)LucF4; R1
 p(−186 to +432)LucF4; R3: 5′-CCGCTCGAGTCCCGTAGAGCATCCTAGCG-3′
 p(−186 to +391)LucF4; R4: 5′-CCGCTCGAGCTTGACCTAGCTTCATTGGAG-3′
 p(−186 to +371)LucF4; R5: 5′-CCGCTCGAGGTTGCCTCTGTTCTTTCTAG-3′
 p(−186 to +227)LucF4; R6: 5′-CCGCTCGAG AATTTCTGCAGCCCGGTGAT-3′
 siRNAF: 5′- GGATGGTTCTGGTCAAATACA-3′
 ScrambledF: 5′- GGGTGCATATACAGATTGACT-3′
Probes for site-directed mutagenesis and EMSA
 p(−186 to 432)Luc mut5′-CGAGTCCCGTAGAGCATCCTAGCGCCCCTGATGAACTTGACCTAGCTTCATTGGAGTTGCCTCTG-3′
 DsbA-L5′-GTCAAGTTCGGGGAGGGGGATCAG-3′
 Sp1C5′-GGGTCTGGGCGGGGGGGAGGGGACC-3′
Primers for ChIP
 DsbA-LF: 5′-CAGAGGCAACTCCAATGAAG-3′; R: 5′-CTAAGTGCTGGACAGAGATG-3′
Primers for quantitative real-time PCR
 DsbA-LF: 5′-GAATGTCCACAGCGCAAGCC-3′; R: 5′-AGTGGTGGGTAGCCCAAAGG-3′
 GAPDHF: 5′-GGATTTGGCCGTATTGGG-3′; R: 5′-GTTGAGGTCAATGAAGGGG-3′
  • NheI and XhoI restriction sites are underlined. mut, mutant; siRNA, small interfering RNA.